-
Ikhithi yokuhlola ye-Elisa ye-CAP
I-Kwinbon le khithi ingasetshenziswa ekuhlaziyeni inani kanye nekhwalithi yensalela ye-CAP emikhiqizweni yasemanzini izimfanzi zezinhlanzi njll.
Idizayinelwe ukuthola i-chloramphenicol ngokususelwe kumgomo “wokuncintisana okuqondile” kwe-enzyme immunoassay.Imithombo ye-microtiter ihlanganiswe ne-antigen ehlangene.I-Chloramphenicol kusampula iqhudelana ne-antigen enamathelayo ukuze ibophe inani elilinganiselwe le-antibody elengeziwe .Ngemva kokwengezwa kwesu elilungele ukusetshenziswa le-TMB isiginali ikalwa ngesifundi se-ELISA.Ukumuncwa kuhambisana ngokuphambene nokugxilisana kwe-chloramphenicol kusampula.
-
Ikhithi Yokuncintisana Ye-Enzyme Immunoassay Yokuhlaziya Okuningi kwe-Tylosin
I-Tylosin iyi-antibiotic ye-macrolide, esetshenziswa kakhulu njenge-antibacterial kanye ne-anti-mycoplasma.Ama-MRL aqinile awakasungulwa njengoba lesi sidakamizwa singase siholele kumphumela omubi emaqenjini athile.
Le khithi ingumkhiqizo omusha osuselwe kubuchwepheshe be-ELISA, obusheshayo, obulula, obunembile futhi obuzwelayo uma uqhathaniswa nokuhlaziywa kwezinsimbi okuvamile futhi udinga amahora angu-1.5 kuphela ekusebenzeni okukodwa, kunganciphisa kakhulu iphutha lokusebenza kanye nokuqina komsebenzi.
-
Ikhithi Yokuncintisana Ye-Enzyme Immunoassay Yokuhlaziya Okuningi kwe-Flumequine
I-Flumequine iyilungu le-quinolone elwa namagciwane, esetshenziswa njenge-anti-infective ebaluleke kakhulu emkhiqizweni wezilwane kanye nowasemanzini ngenxa yobubanzi bayo, ukusebenza kahle okuphezulu, ubuthi obuphansi kanye nokungena kwezicubu eziqinile.Ibuye isetshenziselwe ukwelapha izifo, ukuvimbela kanye nokukhuthaza ukukhula.Ngoba kungaholela ekuphikisweni kwezidakamizwa kanye nokuba khona kwe-carcinogenicity, umkhawulo ophezulu lapho ngaphakathi kwezicubu zezilwane unqunywe e-EU, eJapane (umkhawulo ophezulu ngu-100ppb e-EU).
Njengamanje, i-spectrofluorometer, i-ELISA kanye ne-HPLC izindlela eziyinhloko zokuthola izinsalela ze-flumequine, futhi i-ELISA ibe yindlela evamile yokuzwela okuphezulu nokusebenza okulula.
-
Ikhithi Yokuhlola ye-Elisa ye-AOZ
Le kit ingasetshenziswa ekuhlaziyweni komthamo kanye nekhwalithi yensalela ye-AOZ ezicutshini zezilwane (inkukhu, izinkomo, ingulube, njll), ubisi, uju namaqanda.
Ukuhlaziywa kwezinsalela zezidakamizwa ze-nitrofuran kudinga ukusekelwe ekutholeni ama-metabolites aboshwe ngezicubu zezidakamizwa zomzali we-nitrofuran, okuhlanganisa i-Furazolidone metabolite (AOZ), i-Furaltadone metabolite (AMOZ), i-Nitrofurantoin metabolite (AHD) ne-Nitrofurazone metabolite (SEM).
Uma kuqhathaniswa nezindlela zechromatographic, ikhithi yethu ibonisa izinzuzo ezinkulu mayelana nokuzwela, umkhawulo wokutholwa, okokusebenza kobuchwepheshe kanye nemfuneko yesikhathi. -
Ikhithi Yokuhlola ye-Elisa ye-Ochratoxin A
Le khithi ingasetshenziswa ekuhlaziyeni inani nekhwalithi ye-ochratoxin A ekuphakeleni.Ungumkhiqizo omusha wokutholwa kwezinsalela zomuthi ngokusekelwe kubuchwepheshe be-ELISA, obiza kuphela amaminithi angu-30 ekusebenzeni ngakunye futhi onganciphisa kakhulu amaphutha okusebenza kanye nokuqina komsebenzi.Le khithi isuselwe kubuchwepheshe bokuncintisana obungaqondile be-ELISA.Imithombo ye-microtiter ihlanganiswe ne-antigen ehlangene.I-Ochratoxin A kusampula iqhudelana ne-antigen ehlanganiswe epuleti le-microtiter ye-antibody eyengezwe .Ngemuva kokwengezwa kwe-enzyme conjugate, i-substrate ye-TMB isetshenziselwa ukukhombisa umbala.Ukungabi khona kwesampula kuhlobene kabi nensalela ye-o chratoxin A kuyo, ngemva kokuqhathanisa Nejika Elijwayelekile, eliphindaphindwa izici zokuhlanjululwa, inani le-Ochratoxin A kusampula lingabalwa.
-
Ikhithi Yokuhlola ye-Elisa ye-Aflatoxin B1
I-Aflatoxin B1 ikhemikhali enobuthi ehlala ingcolisa okusanhlamvu, ummbila namakinati, njll. Umkhawulo oqinile wezinsalela usumisiwe we-aflatoxin B1 ekudleni kwezilwane, ekudleni namanye amasampula.Lo mkhiqizo usekelwe ku-ELISA yokuncintisana engaqondile, esheshayo, enembayo futhi ezwelayo uma iqhathaniswa nokuhlaziywa kwezinsimbi okuvamile.Idinga kuphela imizuzu engama-45 ekusebenzeni okukodwa, okunganciphisa kakhulu iphutha lokusebenza kanye nokuqina komsebenzi.
-
Ikhithi Yokuhlola ye-Elisa ye-AMOZ
Le khithi ingasetshenziswa ekuhlaziyeni inani nekhwalithi yensalela ye-AMOZ emikhiqizweni yasemanzini (izinhlanzi nezimfanzi), njll. Ukuhlolwa kokuhlolwa kwe-enzyme, uma kuqhathaniswa nezindlela ze-chromatographic, kubonisa izinzuzo ezinkulu mayelana nokuzwela, umkhawulo wokuthola, okokusebenza kobuchwepheshe kanye nemfuneko yesikhathi.
Le khithi yakhelwe ukuthola i-AMOZ ngokusekelwe kumgomo wokuhlolwa kwe-enzyme engaqondile yokuncintisana.Imithombo ye-microtiter imbozwe nge-capture BSA exhunywe
i-antigen.I-AMOZ kusampula iqhudelana ne-antigen embozwe epuletini le-microtiter ye-antibody engeziwe.Ngemuva kokwengezwa kwe-enzyme conjugate, i-substrate ye-chromogenic iyasetshenziswa futhi isignali ikalwa nge-spectrophotometer.Ukumuncwa kuhambisana ngokuphambene nokugxilisana kwe-AM OZ kusampula.
