umkhiqizo

I-2.Izidakamizwa ze-nitrofuran i-furaltadone, i-nitrofurantoin ne-nitrofurazone zavinjelwa ukusetshenziswa ekukhiqizeni izilwane zokudla e-EU ku-1993, futhi ukusetshenziswa kwe-furazolidone kwakuvinjelwe ku-1995. ama-metabolites abophekile ezidakamizwa zomzali we-nitrofuran, njengoba izidakamizwa zabazali zishintshwa ngokushesha kakhulu, futhi ama-metabolites e-nitrofuran aboshwe ngezicubu azogcinwa isikhathi eside, ngakho-ke ama-metabolites asetshenziswa njengokuhlosiwe ekutholeni ukuhlukunyezwa kwe-nitrofuran.I-Furazolidone metabolite (AMOZ), i-Furaltadone metabolite (AMOZ), i-Nitrofurantoin metabolite (AHD) kanye ne-Nitrofurazone metabolite (SEM).

Imininingwane

1.Ikhithi Yokuhlola ye-Elisa ye-AMOZ

2.Ikati.Imithombo ye-KA00205H-96

Izingxenye ze-3.Kit
● Ipuleti le-Microtiter elinemithombo engu-96 embozwe nge-antigen
● Izixazululo ezijwayelekile(6 amabhodlela)
0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb
● Isixazululo esijwayelekile se-spiking: (1ml/ibhodlela) ……………………………………………….100ppb
● I-enzyme conjugate 1ml………………………………………………………..…………….ikepisi elibomvu
● Isixazululo se-antibody 7ml …………………………………………………..………….….ikepisi eliluhlaza
● isixazululo A 7ml……………………………………………………………………………………ikepisi elimhlophe
● isixazululo B 7ml…………………………………………………………….………..…… ikepisi elibomvu
● isixazululo sokumisa 7ml ……………………………………………………….……………ikepisi eliphuzi
● 20×isixazululo sokugeza esigxilile esingu-40ml……………………………………….……ikepisi esobala
● 2×isixazululo sokukhipha okugxilisiwe 50ml………………………………………….…….ikepisi eliluhlaza okwesibhakabhaka
● 2-Nitrobenzaldehyde 15.1mg……………………………………………………….……ikepisi elimhlophe

4.Ukuzwela, ukunemba nokunemba
Ukuzwela: 0.05ppb
Umkhawulo wokutholwa
Imikhiqizo yasemanzini(izinhlanzi nezimfanzi)………….………… 0.1ppb
Ukunemba
Imikhiqizo yasemanzini(izinhlanzi nezimfanzi)…………………… 95±25%
Ukunemba: I-CV yekhithi ye-ELISA ingaphansi kuka-10%.

5.Cross Rate
I-Furaltadone metabolite(AMOZ)…………………………………………………100%
I-Furazolidone metabolite(AMOZ)……………………………..………………..<0.1%
I-Nitrofurantoin metabolite(AHD)………………………………………………<0.1%
I-Nitrofurazone metabolite(SEM)…………………………………………..…<0.1%
Furaltadone……………………………………………………………….…….11.1%
I-Furazolidone………………………………………………………….….…..…<0.1%
I-Nitrofurantoin……………………………………………………………….…<1%
I-Nitrofurazone………………………………………………………………..…<1%